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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is performing quality control on antimicrobial susceptibility testing. The zone diameter for Escherichia coli ATCC 25922 tested against ciprofloxacin is consistently smaller than the acceptable range published by CLSI. The MOST likely cause of this discrepancy is:

A) All of the above
B) Use of expired antibiotic disks
C) Inoculation with a bacterial suspension that is too dense
D) Incubation at an incorrect temperature

2. An elderly patient in a long-term care facility develops a localized outbreak of painful vesicles along a dermatome. Tzanck smear of a vesicle reveals multinucleated giant cells. The MOST likelycausative agent is:

A) Coxsackievirus
B) Varicella-zoster virus
C) Herpes simplex virus type 1
D) Orf virus

3. A 30-year-old female who recently returned from a hiking trip in the northeastern United States presents to her physician with a two-week history of a slowly expanding, erythematous rash with central clearing on her thigh. She also reports intermittent fatigue, arthralgias, and mild headache. Serological testing reveals a positive IgM and IgG response to Borrelia burgdorferi. Which of the following mechanisms is MOST significant in the pathogenesis of the clinical manifestations observed in this patient?

A) Evasion of phagocytosis through a thick polysaccharide capsule
B) Production of potent exotoxins leading to direct tissue damage
C) Induction of a strong pro-inflammatory cytokine response by the host
D) Intracellular replication within macrophages leading to disseminated infection

4. A microbiology laboratory is asked to identify the causative agent of a fungal keratitis. Microscopic examination of corneal scrapings reveals broad, aseptate hyphae with right-angle branching. The MOST likely genus is:

A) Fusarium
B) Aspergillus
C) Candida
D) Mucor or Rhizopus

5. For epidemiological tracking and outbreak investigations of Clostridioides difficile infection (CDI), which molecular typing method based on PCR amplification targeting repetitive elements within the pathogenicity locus (PaLoc) has historically been widely used and designated by specific nomenclature (e.g., NAP1/BI
/027)?

A) PCR Ribotyping
B) Whole-genome sequencing (WGS) SNP analysis
C) Pulsed-field gel electrophoresis (PFGE)
D) Multilocus sequence typing (MLST)

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